Alt-R CRISPR-Cas9: An optimized genome editing solution
Speaker: Dr. Abdelilah Mekhloufi, Field Application Scientist, Integrated DNA Technologies
Host: Dr. Fabien Cubizolles, University of Basel and Dr. Hendrik Mallin, Head of Sales & Key Accounts, LubioScience GmbH
CRISPR: Unleashing the Potential of Genetic Engineering
CRISPR technology has been a major technological breakthrough, making genome editing easier, faster, more efficient, and more robust than previous methods.
In this seminar you will learn:
• Guidelines when designing a CRISPR experiment.
• Advantages and disadvantages of Cas9 vs Cas12 for gene editing, and their applications.
• gRNA design and formats selection recommendation
• How to optimize cell-editing with high on-target and reduced off-target activity using Alt-R HiFi CRISPR-Cas9 nuclease.
• Considerations to improve HDR (Homology Directed Repair) efficiency for knock-in experiment.
• How chemical modifications for dsDNA donor templates improve the HDR efficiency and reduce blunt insertion events.
• How and where to introduce silent mutations to improve HDR rates
• How Alt-R HDR Enhancer V2 further improves HDR rate.
Following the seminar, Food and Drinks will be served outside the seminar room. We therefore kindly ask you to register for this event.
